serologic study on leptospiral infection in sheep in ahvaz, southwestern iran
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abstract
to investigate the seroprevalence of leptospiral infection in sheep in ahvaz, southwestern iran, bloodsamples were taken from 181 female sheep. sera were stored at -20°c until use. they were initially screenedat serum dilution of 1:100 against six live antigens of leptospira interrogans serovars pomona, canicola,hardjo, ballum, icterohaemorrhagiae, grippotyphosa using the microscopic agglutination test (mat). thesamples were considered positive if ≥ 50% of agglutination of leptospire in a dilution test serum of ≥ l:100were observed. sera with positive results were titrated against reacting antigens in serial two-fold dilutionsfrom l:100 to 1:1600. antibodies against one or more serovars were detected in 27 (14.9%) sera at dilution≥1:100. antibodies against more than one serovar were found in 5 (18.5%) positive sera. among the positivesera, antibodies were most frequent to serovar pomona (43.8%) followed by canicola (21.9%),icterohaemorrhagiae (12.5%), grippotyphosa (9.4%), ballum and hardjo (each of them 6.3%). the results ofthis survey indicate that leptospiral infection is common in sheep in ahvaz and that various serovars concurin the etiology.
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Journal title:
iranian journal of veterinary researchPublisher: shiraz university
ISSN 1728-1997
volume 8
issue 4 2007
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